HPCs play an important role in repair, and have also been correlated with increased severity of chronic liver disease as well as development of hepatocellular carcinoma (HCC) 8, 9, 10, 11.
As such, the identification of HPCs is challenging, and a reliable method which is capable of identifying and quantifying HPCs of varying histological phenotypes is urgently required. The phenotype and distribution of HPCs vary according the liver pathophysiology and severity, and known markers including Pan Cytokeratin, CK19, NCAM and SOX-9 also stain cholangiocytes 4, 5, 6, 7. They are present in the healthy liver at low abundance, residing in the liver stem cell niche termed the “canals of Hering” 2, 3. Histologically, they are small ovoid cells with a high nuclear-to-cytoplasmic ratio. HPCs are a heterogeneous population, expressing immature and intermediate phenotypes of biliary and hepatic lineages 1.
THE INFORM SOFTWARE
This study demonstrates that the InForm software package provides a useful tool for liver disease research, allowing rapid, and objective quantification of the presumptive HPCs and identifies histological features that assist with assessing liver disease severity, and potentially can facilitate diagnosis.
Necroinflammation as evaluated by a pathologist, correlated with InForm outputs (r 2 = 0.8192, p < 0.05). Hepatitis samples were then evaluated both by a pathologist using the Ishak-Knodell scoring system, and by InForm through customised algorithms. InForm was configured to identify presumptive HPCs, CD45 +ve inflammatory cells, areas of necrosis, fat and collagen deposition (p < 0.0001). We examined a cohort of eleven acute hepatitis samples and three non-alcoholic fatty liver disease (NAFLD) samples, stained with HPC markers (GCTM-5 and Pan Cytokeratin), an inflammatory marker (CD45), Sirius Red to detect collagen and haematoxylin/eosin for general histology. In this study we utilise PerkinElmer’s “InForm” software package to semi-automate the scoring of patient liver biopsies, and compare outputs to a pathologist’s assessment. However, it is often impractical to recruit pathologists in large cohort studies. Liver disease research often necessitates the grading of disease severity, and pathologists’ reports are the current gold-standard for assessment. The meeting is open to communication officers working on information and publicity issues in a Managing Authority or Joint Technical Secretariat (European Territorial Co-operation programmes).Hepatic progenitor cells (HPCs) play an important regenerative role in acute and chronic liver pathologies. There will be no possibility to follow it via live streaming (a recording of the plenary will be available afterwards). Please note that this event will only be in person. In case of too many registrations, the national coordinators will have the final say on who will be able to attend based on a quota per delegation and on a first come first served basis.
The theme of the meeting is ‘Strategic Communication’.ĭue to the capacity of the venue, the number of people who can join the event is limited. The network meets twice a year, and the next INFORM EU meeting will be held in person in Évora (Portugal) on 16-18 November 2022. Through an online platform, the members can exchange experiences and good practices, coordinate the communication activities and discuss the strategy to increase the outreach and impact of communication activities. The network’s ultimate goal is to establish stronger cooperation between the Commission and EU programmes under shared management. In addition, the INFORM EU Network covers the Recovery and Resilience Facility, a temporary instrument to help Member States deal with the social and economic consequences of the COVID-19 pandemic and build back better.
The network covers several EU funds in the field of Regional Policy, Social Policy, Home Affairs and Maritime Affairs. INFORM EU is a European network of communication officers responsible for the promotion of EU and Member State investments under shared management.
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